Bombay Phenotype Test

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Watch my 2014 BBGuy video on Bombay for more information.

Bombay phenotype test. It is important to be cautious in predicting the ABO blood type of children based on the phenotypes of their parents. Bombay phenotype was first discovered in Bombay India in 1952. Bombay blood group HH blood group Bombay blood group is the rarest blood group.

The Bombay Phenotype was first reported by Bhende 1952 in Bombay India. The absence of H antigen an d reverse grouping with O cell. Clinical test for Bombay phenotype offered by Intergen Genetic Diagnosis and Research Centre Bombay phenotype 616754 Autosomal recessive FUT1 gene Sequence Analysis-All Coding Exons Postnatal - Tests - GTR - NCBI.

As because in our country there is routine practice of only forward or cell type grouping using finger prick method by voluntary blood donors organization and various blood banks. This is due to the fact that a third antigen H on the surface of red cells can prevent the expected ABO blood type from occurring. Bombay and the similar-sounding Para-Bombay phenotype are closely related.

Match the immunodominant sugar with the corresponding ABO phenotype. Everyone in this group tests as having type O blood no matter what their ABO genes are. The classic Bombay phenotype has been reported in those of Indian descendent.

The classical Bombay phenotype is caused by a Tyr316Ter mutation in the coding region of FUT1. It is quite rare in Caucasian with an incidence of 1 in 250000. This very rare phenotype is generally present in about 00004 about 4 per million of the human population though in some places such as Mumbai.

Blood grouping of patients and blood donors with a standard tube method was carried out and samples identified as rare Bombay phenotype were confirmed by saliva inhibition test. First found in Bombay Mumbai in India hence called Bombay blood group. Both the Bombay and para-Bombay phenotypes are the result of point mutations in the FUT1 gene.

But if they are in the Bombay blood group they will still test as type O. A positive test indicates the presence of the antibody. Bombay persons serum contains very strong anti-H and anti-A -B or -AB.

The classical Bombay phenotype is caused by a Tyr316Ter mutation in the coding region of FUT1. Interpret the following test results Anti-A0 Anti-B0 Anti-D3 A1 cells4 B cells4 Anti-H0. Detection of rare blood group Bombay Oh phenotype patients and management by acute normovolemic hemodilution A simple test like blood grouping should be done with serious intention with incorporation of both forward and reverse grouping so that no patient receives wrong blood leading to fatal hemolysis due to transfusion.

In routine blood testing cell grouping shows characteristics of O group. This essentially renders their antibodies incompatible with all other RBCs except those from another Bombay individual. The higher the number the more agglutination.

In this blood group no A or B antigens are identified on red blood cells or in secretions. In Bombay phenotype both red cells and secretions are deficient in H A and B antigens. Reverse typing uses commercial red blood cells A1 and B cells and patient serum.

The purpose of the. Imagine someone with the A version and the B version of ABO. Basic test with Anti H lectin confirms.

They should have type AB blood. Agglutination scores can be 0 1 2 3 or 4. In Bombay phenotype there is a void of A antigen B Antigen as well as H antigen.

Confirms the presence of Anti H in the patient plasma. So there is tremendous chance of misinterpretation or unexploration of this. It is not D galactose L fucose actylgalctosamine.

Anti-H lectin would agglutinate the RBCs of which ABO Types. In Caucasians the Bombay phenotype may be caused by a number of mutations. The commercial RBCs are added to the patient serum and the individual tubes are checked for agglutination.

The H locus contains four exons that span more than 8 kb of genomic DNA. It is presumed that the H antigen is a precursor carbohydrate from which A and B blood groups are formed. Bombay phenotype and epistasis Homozygous recessive condition at one locus makes the expression of a second locus Have gene to make A or B antigen at one loci But lack genes to produce H substance at other loci which gives you the O phenotype.

By definition that would fit type O blood type. The classic Bombay phenotype is rare blood type which was first reported in 1952 in Bombay India and is associated with the ABO and H blood group systems. If both copies of FUT1 are inactive hh the Bombay phenotype results.

The mutation introduces a stop codon resulting in a truncated enzyme that lacks 50 amino acids at the C-terminal end. Normally if an A blood type mother has an O type child the father is expected to be type O or at least to carry the O allele OO AO or BO genotype. Bombay blood group is different.

This presentation from December 2014 is a close look at the rarely seen but commonly discussed Bombay Phenotype We will discuss the basics of ABO and H an.

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